Figure 2.

Fiber orientation influences osteogenic mesenchymal progenitor cell (hES-MP) behavior. (A) Migration of hES-MPs seeded in center of PCL fibers was assessed at days 5, 10, and 15 using MTT assay (images show MTT-stained scaffolds and arrow indicates aligned fiber orientation). (B–D) hES-MP constructs were cultured with or without dexamethasone (+ Dex) for up to 21 days and assayed at days 7, 14, and 21 for (B) total DNA, and (C,D) ALP activity. hES-MP constructs were imaged for cell nucleus (DAPI in blue) and cytoskeleton (Phalloidin-TRITC in red) at (E) day 3 and (F) day 7 at two depths. Data are mean ± 95% CI for n = 6 (MTT) and n = 9 (DNA and ALP). ^*p < 0.05, and ^***p < 0.001 (two-tailed unpaired t-test: random vs. aligned). Scale bars are 50 μm.