TABLE 2.
Assay method and primer set | DNA fragment profile for strain origina
|
No. of loci | No. of genotypes | Genetic diversity | ||||
---|---|---|---|---|---|---|---|---|
R (n = 48) | J (n = 26) | U (n = 21) | CS (n = 5) | A (n = 1) | ||||
RAMD | ||||||||
B7/10 | A | A | A | A | A | 1 | 1 | 0 |
B7/18 | B | B | B | B | B | 1 | 1 | 0 |
B8/11 | C | C | C | C | C | 1 | 1 | 0 |
B4/14 | D | D (2) | D (4) | D | D | 1 | 1 | 0 |
B6/20 | E | E (2) | E | E | E | 1 | 1 | 0 |
B3/20a | F | F (1) | F (2) | F | F | 1 | 1 | 0 |
B3/20b | G | G | G | G | G | 1 | 1 | 0 |
B3/11 | H | H (1) | H (4) | H | H | 1 | 1 | 0 |
AFLP | ||||||||
TAA-TAA | K (7) | K | −(U) | − | K | 8 | 1 | 0 |
TGC-TGC | L (4) | L | L (1) | L | L | 15 | 1 | 0 |
TGC-CTG | M (3) | M (1) | M | M | M | 12 | 1 | 0 |
PCR | ||||||||
AC10 | S | S | S | S | S | 14 | 1 | 0 |
Overall | 57 | 1 | 0 |
Strain origins: R, Germany; J, Japan; U, United States; CS, reference strains; A, Africa. Values in parentheses are numbers of strains not assayed. −(U), TAA-CTG was used instead of TAA-TAA.