Figure EV1. Generation and characterization of Slc10a1 ^S267F mutant mice.

1. Schematic diagram for the generation of Slc10a1 ^S267F mutant mice.
2. Genotyping of Slc10a1 ^S267F mutant mice. Genomic DNA was extracted from mouse tails, and the specific region covering the mutation was amplified by PCR.
3. Identification of Slc10a1 ^S267F homozygous mice by Sanger sequencing analysis.

Source data are available online for this figure.