Figure EV2. Generation and characterization of Sema7a ^R145W mutant mice.

1. Schematic diagram for the generation of Sema7a ^R145W mutant mice.
2. Genotyping Sema7a ^R145W mutant mice. Genomic DNA was extracted from mouse tails, and the specific region was amplified by PCR.
3. Identification of Sema7a ^R145W homozygous mice by Sanger sequencing analysis.

Source data are available online for this figure.