Figure EV4. The Sema7a ^R145W mutation increases PKCδ/ε phosphorylation in hepatocytes.

• A
  The levels of hepatic PKCδ/ε phosphorylation were significantly higher in Sema7a ^R145W homozygous mice than in heterozygous mice and WT mice. Wild type, wild‐type mice, n = 4; Heterozygote, Sema7a ^R145W heterozygous mice, n = 5; homozygote, Sema7a ^R145W homozygous mice, n = 5. *P < 0.05 versus the WT mice, ^# P < 0.05 versus the heterozygous mutant mice. The data were analyzed by the independent‐samples Student's t‐test and the Mann–Whitney U‐test.
• B, C
  Furthermore, Sema7a ^R145W (human R148W) homozygous mutation increased PKCδ/ε phosphorylation in Sema7a ^R145W mouse primary hepatocytes (B) and human HepG2 cells following transfection with SEMA7A_R148W construct (C).

Source data are available online for this figure.