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. 2021 Sep 20;13(11):e14163. doi: 10.15252/emmm.202114163

Figure EV1. Reporters used for RNA‐tagging experiments and proteins identified by mass spectrometry.

Figure EV1

  • A
    Schematic of PP7‐tagged RNA reporters used in this study and the previously published 3x FLAG (3xF)‐tagged reporters that were used to develop the PP7 reporters.
  • B
    Relative nanoluciferase (nLuc) expression from PP7‐tagged reporters compared to the primary 3x FLAG (3xF)‐tagged reporters showed no significant differences in translational efficiency in HEK293T cells (n = 12 biological replicates). Two‐tailed Student’s t‐test with Welch’s correction is used for statistical analysis.
  • C
    Venn diagrams indicate total number of proteins identified for AUG‐nLuc and CGG‐nLuc RNA‐tagging reporters without or with TG treatment (TG+).
  • D
    GO term analysis of manually curated differentially enriched CGG‐interacting proteins.

Source data are available online for this figure.