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. 2021 Sep 20;13(11):e14163. doi: 10.15252/emmm.202114163

Figure 4. SRPK1 knockdown modifies CGG and GGGGCC repeat‐associated toxicity in Drosophila .

Figure 4

  • A, B
    Representative (A) photographs of fly eyes and (B) quantitation with siRNA‐mediated knockdown of SRPK1 (dSRPK1); n = 30–34/genotype. Error bars represent mean ± SD.
  • C
    Representative photographs of fly eyes expressing GMR‐GAL4‐driven (GGGGCC)28‐EGFP with siRNA‐mediated knockdown of SRPK1 or disruption by insertion.
  • D
    Quantitation of rough eye phenotypes. t‐test with Welch corrections for comparisons with the control; n = 31–34 flies/ genotype. Error bars represent mean ± SD.
  • E
    Representative external eye imaging for the detection of GFP aggregates caused by (CGG)90‐EGFP transgene expression (top). Converted images used to quantify total intensity of GFP puncta (bottom).
  • F
    Depletion of SRSF1 or SRPK1 by RNAi results in reduced (CGG)90‐EGFP puncta compared to control siRNA as quantified by total intensity (a.u. = arbitrary unit). n = 13–15 flies/genotype. Error bars represent mean ± SD.

Data information: Statistical analysis was performed using two‐tailed Student’s t‐test with Welch’s correction, ***P < 0.001; ****P < 0.0001.

Source data are available online for this figure.