Figure EV1. Senescence affects endothelial cells in glomeruli and increases with age.

• A, B
  pH2AX/griffonia simplicifolia coimmunostaining (left panels) and quantification (right panel) in kidneys from (A) young and aged mice and (B) 12‐month‐old irradiated and non‐irradiated mice. Quantification represents the number of pH2AX foci per glomerular endothelial cell. Original magnification ×630. Scale bar = 10 μm. Panels are representative images of four mice in each group. Data are means ± SEM. Statistical analysis: Student's t‐test.