Figure EV4. Recombinant PAI‐1 induces podocyte cytoskeleton modifications and detachment.

1. Immunofluorescence of paxillin (green) and phalloidin (red) on podocytes stimulated for 30 min with recombinant PAI‐1 at 5 nM. Quantification of focal adhesions (right panel) was performed by counting the number of paxillin‐positive spots in 30 cells from three independent experiments. Original magnification ×1,000. Scale bar = 20 μm.
2. Podocyte morphology (left panels) and quantification of podocyte detachment (right panel) 30 min after stimulation by recombinant PAI‐1 at 5 nM. Original magnification ×400, n = 4 independent experiments.

Data information: Data are means ± SEM. Statistical analysis: Student's t‐test.