Skip to main content
. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: J Hepatol. 2021 Jan 27;74(6):1429–1441. doi: 10.1016/j.jhep.2021.01.028

Figure 6. Molecular species of BAs in NASH-HCC models and their impact in expression of genes involved in self-renewal, stemness and inflammation.

Figure 6.

A) Heatmap of individual species of BAs measured in livers from AD-Stard1 mice and Stard1 ΔHep mice following DEN treatment and HFHC feeding, showing an increase (red) or a reduction (green) respect to the mean of AD-control and Stard1f/f mice. Values are Log2 of the fold change.

B) Quantification of the unconjugated BAs in liver tissue from each group of animals. (N=5 for AD-Ctrl/AD-Stard1 and N=6 for Stard1f/f and Stard ΔHep mice.

C) Quantification of tauroconjugated BAs in liver tissue from each group of animals.

D) mRNA levels of genes involved in self-renewal, stemness and inflammation in TICs following incubation with CA (50 μM), βMCA (50 μM) and TCA (200 μM) for 48 hours. Values are mean ± SEM. N=3 independent experiments performed in triplicates.

E) Effect of the combination of CA, βMCA and TCA in PMH for 24 hours on the mRNA levels of genes involved in self-renewal, stemness and inflammation.

Values are mean ± SEM. N=3 independent experiments performed in quadruplicates.