Fig. 8.

IMP2 stabilized ZFAS1 promotes the progression of CRC by promoting the ATPase activity of OLA1. ① The critical KH3-4 domain of m⁶A modulator IMP2 directly recognized the specific m⁶A motif (“RGGAC/RRACH”) of ZFAS1, promoted its stability and expression in an m⁶A dependent manner. ② The IMP2 stabilized ZFAS1 subsequently directly bound to the OBG-type functional domain of the OLA1 protein to fully expose the ATP binding site of the OLA1 protein, and ultimately enhanced the ATPase activity of OLA1 protein. ③ The ZFAS1 activated OLA1 enhanced ATP-hydrolysis capacity and activated Warburg effect and ultimately promoted the progression of CRC