Table 1.
List of primers and thermal condition used for molecular identification of Campylobacter species
| Primer | Sequence (5′-3′) | Target | Amplicon size (bp) | PCR condition (30 cycle) | References | ||
|---|---|---|---|---|---|---|---|
| Denature | Annealing | Extension | |||||
|
16S9F 16S1540R |
GAGTTTGATCCTGGCTC AAGGAGGTGATCCAGCC |
16S rRNA | 1530 | 94 °C, 30 s | 47 °C, 30 s | 72 °C, 90 s | Samosornsuk et al. [21] |
|
HIP400F HIP1134R |
GAAGAGGGTTTGGGTGGTG AGCTAGCTTCGCATAATAACTTG |
hipO gene | 735 | 94 °C, 30 s | 55 °C, 30 s | 72 °C, 45 s | Linton et al. [22] |
|
Cj-cdtCU1 Cj-CdtCR2 |
TTTAGCCTTTGCAACTCCTA AAGGGGTAGCAGCTGTTAA |
Cj-cdtC | 524 | 94 °C, 30 s | 53 °C, 30 s | 72 °C, 30 s | Asakura et al. [23] |
|
Cc-CdtCU1 Cc-CdtCR1 |
TAGGGATATGCACGCAAAG GCTTAATACAGTTACGATAG |
Cc-cdtC | 313 | ||||
|
CfspCU2 CfspCR1 |
AAGCATAAGTTTTGCAAACG GTTTGGATTTTCAAATGTTCC |
Cf-cdtC | 397 | ||||
Cj: C. jejuni; Cc: C. coli; Cf: C. fetus