Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 8;500:113182. doi: 10.1016/j.jim.2021.113182

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Elsevier B.V. All rights reserved.

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

PMC Copyright notice

Fig. 1 — RBD protein production.

SDS-PAGE of elution fractions containing RBD. Recombinant RBD was eluted from the immobilized metal affinity chromatography (IMAC) with increasing concentrations of imidazole. (A) Coomasie brilliant blue stain of protein fractions obtained from cells grown in complete medium (A) or reduced-serum medium (B), (C) Western blot of protein fractions in B using anti-his as primary antibody (GE) 1/1000 and anti mouse as secondary antibody (Sigma) 1/30,000. Imidazole concentrations used to elute the proteins are indicated in x axis. MWM: 14–100 kDa Blue Plus Protein Marker (TransGen Biotech).