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Validation of in-house IgG ELISA using RBD.
ELISA for detection of SARS-CoV-2–specific IgG antibodies. (A) Blue dots indicate serum reactivity to RBD and red dots indicate serum reactivity to S. The dotted line indicates ELISA cut-off value = 0.1 (mean of negative sera +3xSD) and 0.25 for IgG RBD ELISA and COVIDAR, respectively. (B) Spearman's rank correlation analysis between the OD values obtained by the in-house RBD ELISA (y) and COVIDAR (x). R = 0.84, p-value <2.2 e-16.
