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. Author manuscript; available in PMC: 2022 Sep 27.
Published in final edited form as: J Chem Inf Model. 2021 Aug 13;61(9):4224–4235. doi: 10.1021/acs.jcim.1c00683

Figure 2.

Figure 2.

MicroScale Thermophoresis binding analysis for the interaction between Spike RBD and lumefantrine. The concentration of labeled Spike RBD was maintained at 5 nM, and the ligand concentration varied from 125 μM to 3.8 nM. The serial titrations result in measurable changes in the fluorescence signal within a temperature gradient that was used to calculate the dissociation constant (Kd = 259 ± 78 nM). The curve is shown as Fraction Bound [-] against lumefantrine concentration on a log scale.