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. 2021 Oct 18;45(5):588–598. doi: 10.3906/biy-2104-4

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Copyright © 2021 The Author(s)

This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use and redistribution provided that the original author and source are credited.

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The strategy of Bex2 knock out and screening. (A) Graphical representation of the Bex2 gene and targeting sites. Exon-3 is targeted for deletion by designing two sgRNAs. Forward and reverse primers flanking the two sgRNAs sites were intended for genotyping. (B) Genotyping of the pups by PCR. Two pups (#18 and #19) showed knocked-out bands (Bex2-/-). (C) Sanger sequencing chromatogram of the deletion site. Arrow indicates the deletion joint. (D) qRT-PCR confirmation of Bex2 mRNA loss in brain and lungs.