FIG. 2.

(A) Agarose gel electrophoresis of genomic DNA from various PLC-producing bacteria. (B) Southern blot of DNA from panel A, probed with the labeled 4.4-kb DNA insert from pSN-1. Lanes: 2, B. pseudomallei; 3, B. mallei; 4, B. cepacia; 5, P. aeruginosa; 6, L. monocytogenes; 7, C. perfringens; 8, Bacillus cereus. A standard lambda/HindIII DNA marker and Salmonella paratyphi A genomic DNA (negative control) were included in lanes 1 and 9, respectively. The positions of molecular size markers are shown on the left of both panels.