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. 2021 Oct 25;12:754823. doi: 10.3389/fpls.2021.754823

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Copyright © 2021 Jin, Chao, Li, Wang, Cheng, Li and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transient silencing of TaFBN and Ta_Pes_BRCT with the BSMV-VIGS method. (A) Wheat leaves treated with 1× Fes buffer (MOCK) show no phenotypic changes. Mild chlorotic mosaic symptoms were detected on the plants inoculated with BSMV:γ, BSMV:PDS, BSMV:TaFBN, or BSMV:Ta_Pes_BRCT. (B) Phenotypes of the fourth leaves infected with uredospores of Pst race CYR33 at 10 days post inoculation. (C) Silencing efficiency assessment of TaFBN in the TaFBN-knockdown plants and Ta_Pes_BRCT in the Ta_Pes_BRCT-knockdown plants. Error bars represent the standard deviations of three independent samples. The significance of differences is indicated by asterisks and tested using Student's t-test (P < 0.05).