Figure 4.

Histological observations of Pst race CYR33 infection in TaFBN-knockdown and Ta_Pes_BRCT-knockdown wheat plants. (A) WGA was used to stain the leaves to visualize pathogens. (B) Number of HB/HMC/H; (C) length of IH; (D) infection unit area; (E) H₂O₂ area; and (F) necrotic area staining by 3,3′-diaminobenzidine (DAB) were measured with DP-BSW software in TaFBN-knockdown plants at 48 and 120 hpi after inoculation. SV, substomatal vesicle; IH, initial hyphae; HMC, haustorial mother cell; SH, secondary hyphae. Error bars represent the standard deviations of three independent samples. The significance of differences is indicated by asterisks and tested using Student's t-test (P < 0.05). Bar, 50 μm.