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. 2021 Oct 25;12:754823. doi: 10.3389/fpls.2021.754823

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Copyright © 2021 Jin, Chao, Li, Wang, Cheng, Li and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Stable transformation of the susceptible bread wheat cultivar Fielder with TaFBN confers resistance to Pst race CYR33. (A) Identification by PCR of individuals transformed with TaFBN and Ta_Pes_BRCT. Wild-type Fielder was used as a negative control; M8664-3 containing the full sequence of TaFBN and Ta_Pes_BRCT was used as a positive control. (B) Fielder T₂ plants transformed with TaFBN were resistant to CYR33 but those transformed with Ta_Pes_BRCT were susceptible to CYR33. Wild-type Fielder, Chinese Spring, and M8664-3 were used as susceptible and resistant controls.