FIGURE 10.
HSP70‐driven signalling in TAM. (A) Representative immunoblots showing induction of nuclear translocation of p65 by rhHSP70. NE: nuclear extracts; CE: cytosolic extracts. ascTAM were pre‐incubated for 2 h with 10 μg/ml polymyxin B (PMB) before 1 μg/ml low‐endotoxin rhHSP70 was added for 2.5 h. The control (Ctrllow) was spiked with the amount of LPS present in rhHSP70 as contaminant. (B) Quantification of nuclear translocation of p65 (relative to GAPDH, n = 6). (C) Representative immunoblot of whole cell lysates showing induction of IKKα/β (panel A) by rhHSP70. Conditions were as in panel A, except that rhHSP70 was added 30 min. (D) Quantification of IKKα/β expression (relative to GAPDH, n = 6). Each data point represents TAM from different patients. (E) Quantification of IL‐6 secretion by TAM after 24 h of rhHSP70 stimulation. IL‐6 in culture supernatants of TAM stimulated as described above were quantified by ELISA (n = 4 TAM). Horizontal bars indicate means. Asterisks indicate p values determined by two‐sided, paired t‐test. *p < .05, **p < .01