Figure 1.

Plasma H₂S production capacity and sulfide levels are reduced in patients with vascular disease, with production capacity predicting mortality. (A) Plasma H2S production capacity and (B) plasma sulfide measured by the MBB method from human patients suffering vascular occlusive disease including 115 patients undergoing carotid endarterectomy (CEA), infra-inguinal revascularization (IGR) and amputation (Amp), as well as age-matched individuals (n = 20). Error bars indicate SD; different letters indicate difference p < 0.05 in One Way ANOVA Tukey post-hoc test. (C) Correlation between H₂S production capacity and sulfide measured by the MBB method in vascular disease patients. (D) Probability of survival for vascular disease patients during follow up after intervention with low (n = 57) vs. high (n = 57) H₂S production capacity and (E) low and high plasma sulfide measurements. High vs. low was determined by median split. P value calculated from log-rank test.