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. 2021 Oct 25;8:750926. doi: 10.3389/fcvm.2021.750926

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Copyright © 2021 Longchamp, MacArthur, Trocha, Ganahl, Mann, Kip, King, Sharma, Tao, Mitchell, Ditrói, Yang, Nagy, Ozaki, Hine and Mitchell.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mechanisms of H₂S release from plasma. (A) Correlation between plasma cystathionine-beta synthase activity and plasma H₂S release. (B) Correlation between plasma H₂S release performed under protein non-denaturing (x-axis) and protein denaturing (y-axis) conditions. (C) H₂S release from plasma when incubated with PLP, PLP + EDTA or PLP + EGTA. (D) In vitro H₂S release performed using cysteine and increasing concentration of FeCl₃, (E) PLP or (F) homocysteine (n = 5 reactions per condition). *p < 0.05, ****p < 0.0001 vs. respective 0uM control.