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. Author manuscript; available in PMC: 2021 Nov 8.
Published in final edited form as: Cell Rep. 2021 Jun 22;35(12):109285. doi: 10.1016/j.celrep.2021.109285

Figure 2. Anti-PD-1 increased antitumor immunity in the tumor microenvironment.

Figure 2.

Flow cytometric analysis of tumors from obese mice treated with IgG2a or anti-PD-1 was conducted to quantify frequencies of immune cell infiltrates.

(A) Tumor dendritic cells (tDCs).

(B) Tumor macrophages (tMacs).

(C) M1-like and M2-like tMac subtypes.

(D) M1-like/M2-like ratio of tMacs.

(E) CD3+ T cells.

(F) CD3+ T cell staining by immunofluorescence and quantification in 6 20× fields. Representative 20× images are shown. Scale bar is 200 μM. DAPI nuclear stain shows negative control without primary antibody.

(G) CD8+ and CD4+ T cells.

Data are shown as mean ± SEM. *p < 0.05 and **p < 0.01 by two-way ANOVA. n = 5–8. See also Figures S1 and S2.