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. Author manuscript; available in PMC: 2021 Nov 8.
Published in final edited form as: Cell Rep. 2021 Jun 22;35(12):109285. doi: 10.1016/j.celrep.2021.109285

Figure 4. Immune checkpoint blockade reprograms immunosuppressive splenic immune cells induced by obesity.

Figure 4.

Flow cytometric analysis of spleens from lean and obese tumor-free mice (black diamond and green X) and lean and obese tumor-bearing mice treated with IgG2a or a-PD-1 (black circle and red square) is presented.

(A and B) Splenic myeloid-derived suppressor cells (sMDSCs).

(C–F) sMDSCs positive for MHCII (C and D) or immune checkpoint PD-L1 (E and F).

(G and H) Monocytic (M) and polymorphonuclear (PMN, P) subtypes of sMDSCs.

(I and J) Splenic DCs (sDCs).

(K and L) Splenic Macs (sMacs).

(M and N) M1-like and M2-like sMac subtypes.

(O and P) Frequency of PD-L1+ sMacs.

Data are shown as mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001 by two-way ANOVA. n = 8–10. See also Figure S2B.