| Antibodies |
|
|
|
| RedFluor 710 anti-Human/Mouse CD11b (M1/70) Antibody |
Tonbo Biosciences |
80-0112 RRID: AB_2621979 |
| VioletFluor 450 anti-Mouse CD45 (30-F11) |
Tonbo Biosciences |
75-0451 RRID:AB_2621947 |
| FITC anti-Mouse CD8a (53-6.7) antibody |
Tonbo Biosciences |
35-0081 RRID:AB_2621671 |
| PE anti-Mouse F4/80 Antigen (BM8.1) antibody |
Tonbo Biosciences |
50-4801 RRID:AB_2621795 |
| PerCp Cy5.5 anti-Mouse Ly-6G (1A8) antibody |
Tonbo Biosciences |
651276: RRID:AB_2621899 |
| APC anti-mouse Ly-6C antibody |
Biolegend |
Cat# 128015, RRID:AB_1732087 |
| Brilliant Violet 711 anti-mouse CD274 (B7-H1, PD-L1) antibody |
Biolegend |
124319, RRID:AB_2563619 |
| Brilliant Violet 605 anti-mouse CD4 antibody |
Biolegend |
100548, RRID:AB_2563054 |
| Brilliant Violet 421 anti-mouse CD279 (PD-1) antibody |
Biolegend |
Cat# 109121, RRID:AB_2687080 |
| Brilliant Violet 650 anti-mouse CD11c antibody |
Biolegend |
Cat# 117339, RRID:AB_2562414 |
| Brilliant Violet 785 anti-mouse CD3epsilon antibody |
Biolegend |
Cat# 100355, RRID:AB_2565969 |
| PE/Cy 7 anti-mouse I-A/I-E antibody |
Biolegend |
Cat# 107629, RRID:AB_2290801 |
| Anti-Mouse CD16 / CD32 (2.4G2) antibody |
Tonbo Biosciences |
Cat# 70-0161, RRID:AB_2621487 |
| Alexa Fluor 488 anti-mouse Gr-1 antibody |
Biolegend |
Cat# 108419, RRID:AB_493480 |
| Anti-PD-1 antibody |
Bio X Cell |
BE0146, RRID:AB_10949053 |
| rat IgG2a isotype control antibody |
Bio X Cell |
Cat# BE0089, RRID:AB_1107769 |
|
| Chemicals, peptides, and recombinant proteins |
|
|
|
| Corning Matrigel Membrane Matrix HC: |
Corning |
354263 |
| UltraComp eBeads |
Thermo Fisher |
01-2222-41 |
| ArC Amine Reactive Compensation Bead Kit |
Thermo Fisher |
A10628 |
| Flow Staining Buffer (1X) |
Tonbo biosciences |
TNB-4222-L500 |
| Foxp3 / Transcription Factor Fix/Perm Concentrate (4X) |
Tonbo biosciences |
TNB-1020-L050 |
|
| Critical commercial assays |
|
|
|
| NCBI Geo (GSE) - GSE174055 SuperSeries |
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE174055
|
Immune checkpoint blockade reprograms systemic immune landscape and tumor microenvironment in obesity-associated breast cancer |
| NCBI Geo (GSE) - Tumor GSE174053 SubSeries |
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE174053: |
Immune checkpoint blockade reprograms systemic immune landscape and tumor microenvironment in obesity-associated breast cancer [tumor] |
| NCBI Geo (GSE) - Tumor Adjacent Adipose in Mammary Fat Pad GSE174044 SubSeries |
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE174044: |
Immune checkpoint blockade reprograms systemic immune landscape and tumor microenvironment in obesity-associated breast cancer [Mammary fat pad, MFP] |
| Human Normal Breast Study (NBS) NCBI Geo GSE50939
|
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE50939. |
Tumor Intrinsic Subtype is Reflected in Cancer-Adjacent Benign Tissue |
|
| Experimental models: Cell lines |
|
|
|
| E0771-luciferase (derived from ATCC CRL-3461. A gift from Dr. Hassan Korkaya, August University, Augusta GA) |
ATCC |
CRL-3461 |
|
| Experimental models: Organisms/strains |
|
|
|
| Mouse: C57BL/6J |
The Jackson Laboratories |
stock number: 000664 |
|
| Software and algorithms |
|
|
|
| ImageJ |
(Schneider et al., 2012) |
https://imagej.nih.gov/ij/
|
| FlowJo Software |
FlowJo |
https://www.flowjo.com/
|
| Timer 2.0 |
Li et al., 2020
|
http://timer.cistrome.org/
|
| CIBERSORT |
Newman et al., 2021
|
https://cibersort.stanford.edu/
|
| xCell |
Aran et al., 2017
|
https://xcell.ucsf.edu/
|
|
| Other - diets |
|
|
|
| High fat diet (60% kcal from fat) |
Research Diets Inc |
D12492i |
| Low fat diet (10% kcal from fat) |
Research Diets Inc |
D12450Ji |
