Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 25;8:753276. doi: 10.3389/fmolb.2021.753276

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Liu, Chen, Huang, Ma, Huang, Wang and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

RPA primer screen for highly sensitive detection. (A) Relative locations of RPA primers to the FLT3-691 site. Forward and reverse primers are colored in yellow and purple, relatively. (B) Comparison of amplification efficiency between different RPA primer pairs. The naked-eye results and fluorescence intensity results are shown above and below, respectively. The relative fluorescence intensity is normalized by setting the highest value to 1. A green triangle points out the most efficient F2R1 pair. (C) The identification of the amplification capacity of F2R1-mediated RPA using 1e7 ∼ 1e1 gradient copies of L691 plasmid templates. This assay was conducted by 15 min of RPA and 15 min of L691-crRNA induced Cas12a reaction, both under 37°C. The fluorescence intensity results and the naked-eye results are shown above and below, respectively. (D) Time-course analysis of (C).