| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit monoclonal anti-TREM2 antibody | Abcam | Cat#ab209814 |
| Biotinylated goat polyclonal anti-TREM2 antibody | R&D systems | Cat#BAF1828; RRID: AB_2208688 |
| Bacterial and Virus Strains | ||
| MACH1 T1 | ThermoFisher | Cat#C862003 |
| DH10Bac | ThermoFisher | Cat#10361012 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| pTT5-SP-TREM2-6xC-His- (19-131) | This paper | N/A |
| pTT5-SP-TREM2-6xC-His-avi-(19-174) | This paper | N/A |
| pHTBV1.1-SecNH-Bio:TREM2-10xN-His-C-avi (19-174) | This paper | N/A |
| pFB-Sec-Bio5:TREM2 N6xHis-C-avi (19-131) | This paper | N/A |
| pFB-Sec-Bio5:TREM2 N6xHis-C-avi (19-138) | This paper | N/A |
| pFB-Sec-NH:TREM2 N-6xHis (19-174) | This paper | N/A |
| TREM2 standard peptide | SinoBiological | Cat#11084-H08H |
| TMB ELISA substrate | ThermoFisher | Cat# 34028 |
| HRP conjugated streptavidin | ThermoFisher | Cat#N100 |
| Alexa Fluor 647 NHS ester | ThermoFisher | Cat#A37573 |
| Deposited Data | ||
| scFv-4 with TREM2 (19-174) | This paper | PDB: 6Y6C |
| scFv-4 with TREM2 (19-131) | This paper | PDB: 6YMQ |
| scFv-2 with TREM2 (19-131) | This paper | PDB: 6YYE |
| SP140 PHD-Bromodomain complex with scFv (data phasing) | Fairhead et al., 2019 | PDB: 6G8R |
| TREM2 Ig-domain (data phasing) | Sudom et al. (2018) | PDB: 5UD7 |
| Experimental Models: Cell Lines | ||
| HEK293 | ATCC | Cat#CRL1573 |
| HEK293 transfected with TREM2-DAP12 | Thornton et al. (2017) | N/A |
| Expi293F (for protein expression) | ThermoFisher | Cat#A14527 |
| Sf9 (for protein expression) | ThermoFisher | Cat#11496015 |
| Oligonucleotides | ||
| List of oligonucleotides used in this study | This study | Table S1 |
| Recombinant DNA | ||
| pHL-sec (Signal peptide origin) | Aricescu et al. (2006) | Addgene plasmid # 99845 |
| pHTBV1.1-SecNH-Bio | Strain-Damerell et al. (2014) | N/A |
| pFB-Sec-Bio5 | SGC | N/A |
| pFB-Sec-NH | SGC | Addgene plasmid # 39189 |
| pTT5 | Zhang et al. (2013) | Addgene plasmid # 52326 |
| Software and Algorithms | ||
| Phenix version 1.17.1 | Emsley et al. (2010); Liebschner et al. (2019) | https://www.phenix-online.org/ |
| ChimeraX version: 0.93 | Goddard et al. (2018) | https://www.rbvi.ucsf.edu/chimerax/ |
| Buster 2.10.3 | Bricogne et al. (2016) | https://www.globalphasing.com/buster/ |
| PyMOL 2.3.4 | The PyMOL Molecular Graphics System, Version 2.3 Schrödinger, LLC | https://pymol.org/2/#download |
| GraphPad Prism version 8.42 for Windows | GraphPad Software, La Jolla California USA | https://www.graphpad.com |
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