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. 2021 Nov 8;16(11):e0259636. doi: 10.1371/journal.pone.0259636

Fig 5. Simultaneous determination of ΔpH and ΔΨ to calculate Δp as a function of respiration rate.

Fig 5

2.5 x 109 K+-depleted cells/ml expressing 3 copies of pH-sensitive GFP were incubated in depletion buffer to which 16.5 mM sodium malate (Mal) was added at the indicated time point followed by 10 mM KCl. In panel A, ΔpH (black) and ΔΨ (red) were determined as described in Methods to calculate Δp (green), shown in panel B overlayed with the O2 concentration trace (blue) showing respiration rates recorded after the indicated additions.