Table 1. Metabolic parameters of P. denitrificans strains grown in glucose or malatea.
| Strain | Doubling time (min) | O2 consumption (mol O2/min/cell) | CO2 generation (mol CO2/min/cell) | RQb | Cytochrome aa3 (mol/cell) |
O2 consumption (mol O2/min/mol cyt aa3) |
|---|---|---|---|---|---|---|
| Wild-type Glucose (n = 5) | 113 ± 0.7 | 3.3 ± 0.5 x 10−17 | 3.3 ± 0.04 x 10−17 | 1.0 | 1.93 ± 0.03 x 10−20 | 1700 ± 30 |
| Wild-type Malate (n = 5) | 81 ± 1 | 4.2 ±0.3 x 10−17 | 6.2 ± 0.2 x 10−17 | 1.5 | 1.89 ± 0.05 x 10−20 | 2230 ± 160 |
| CyoB-/CcoN-Glucose (n = 6) | 106 ± 0.6 | 3.3 ± 0.5 x 10−17 | 3.3 ± 0.5 x 10−17 | 1.0 | 2.30 ± 0.02 x 10−20 | 1450 ± 220 |
| CyoB-/CcoN-Malate (n = 3) | 89 ± 2 | 4.3 ± 0.2 x 10−17 | 7.0 ± 0.9 x 10−17 | 1.6 | 2.31 ± 0.02 x 10−20 | 1850 ± 70 |
Mean ± SE values are shown.
aCells were harvested at an OD of 1.5 with the exception of 4 growths of CyoB-/CcoN- glucose cells grown to 15 OD).
bRespiratory Quotient was calculated by dividing CO2 generation by O2 consumption close to the end of growth when O2 consumption values were more reliable.