Table 8. Concentration of nitrogenated bases, nucleosides and nucleotides in wild-type cells in the absence or presence of 10 mM KCla.
| Metabolite | Concentration (pmol/109 cells)b | Ratioc | ||||
|---|---|---|---|---|---|---|
| Glucose | Glucose + K+ | Malate | Malate + K+ | Glucose K+/no K+ | Malate K+/no K+ | |
| Adenine | 21 ± 1.4 | 21 ± 1.1 | 17 ± 1.9 | 17 ± 1 | 1.01 | 0.98 |
| Adenosine | 12 ± 2.9 | 9.1 ± 0.6 | 6.7 ± 0.2 | 5.8 ± 0.5 | 0.76 | 0.87 |
| AMPd | 76 ±25 (109 ± 10) | 106 ± 83 (103 ± 36) | 57 ± 7 (81 ± 8) | 67 ± 5.2 (85 ± 3) | 1.39 (0.96) | 1.16 (1.06) |
| ADPd | 186 ± 15 (227 ± 18) | 264 ± 52 (241 ± 18) | 140 ± 4.4 (178 ± 4) | 200 ± 6 (105 ± 7) | 1.42 (1.06) | 1.43 (0.59) |
| ATPd | 623 ± 78 (1079 ± 116) | 1690 ± 90 (2620 ± 118) | 451 ± 21 (834 ± 39) | 808 ± 75 (1346 ± 133) | 2.72 (2.43) | 1.79 (1.61) |
| Guanine | 25 ± 2.5 | 27 ± 1.7 | 21 ± 2.4 | 22 ± 1.7 | 1.10 | 1.05 |
| Guanosine | 4.9 ± 0.6 | 6.1 ± 0.5 | 5.4 ± 0.4 | 4.4 ± 0.4 | 1.25 | 0.82 |
| GDP | 93 ± 7 | 165 ± 38 | 64 ± 5.2 | 144 ± 9 | 1.78 | 2.24 |
| GTP | 429 ± 45 (404± 48) | 1,427 ± 112 (1401 ± 65) | 288 ± 13 (303± 14) | 815 ± 84 (819± 80) | 3.33 (3.47) | 2.83 (2.7) |
| Cytosine | 27 ± 2.5 | 28 ± 0.9 | 28 ± 1.1 | 26 ± 1.7 | 1.05 | 0.93 |
| Cytidine | 3 ± 0.3 | 6.4 ± 0.6 | 2.7 ± 0.2 | 4.2 ± 0.3 | 2.16 | 1.52 |
| CTP | 307 ± 52 | 397 ± 23 | 235 ± 28 | 162 ± 12 | 1.29 | 0.69 |
| Thymine | 587 ± 52 | 572 ± 44 | 82 ± 29 | 45 ± 7 | 0.97 | 0.55 |
| Thymidine | 26 ± 3.4 | 26 ± 2.4 | 26 ± 2.6 | 22 ± 1.8 | 1.00 | 0.83 |
| Uracil | 45 ± 11 | 49 ± 2.2 | 67 ± 9 | 59 ± 7 | 1.07 | 0.88 |
| UDP | 65 ± 4.7 | 101 ± 23 | N.D. | 111 ± 3.1 | 1.57 | high |
| UTP | 212 ± 19 | 639 ± 44 | 90 ± 3.6 | 417 ± 32 | 3.01 | 4.65 |
| Hypoxanthine | 5.2 ± 0.6 | 8.8 ± 1.3 | 13 ± 2.4 | 13 ± 2.1 | 1.70 | 0.98 |
| Inosine | 1.0 ± 0.1 | 1.5 ± 0.1 | 2.1 ± 0.5 | 1.5 ± 0.4 | 1.39 | 0.73 |
| NADH + NAD+ | 351 ± 15 | 383 ± 15 | 190 ± 21 | 140 ± 16 | 1.09 | 0.73 |
| NADPH + NADP+ | 127 ± 7 | 106 ± 7 | 92 ± 6 | 62 ± 7 | 0.84 | 0.67 |
aMetabolites with statistically significant differences (p < 0.05; Welch’s t-test) between the presence and absence of K+ in at least one substrate (glucose or malate) are shaded orange, as are the respective concentration values and ratios.
bMean ± SE of 3–5 experiments. N.D. = below detection limit. Metabolite concentration can also be expressed in μM assuming an intracellular volume of 1 μl/109 cells.
cA “high” ratio indicates that the metabolite was not detected in the substrate only condition.
dValues in parentheses were determined separately by HPLC/UV quantitation of adenine nucleotides.