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. Author manuscript; available in PMC: 2022 Oct 29.
Published in final edited form as: Circ Res. 2021 Sep 23;129(10):e202–e214. doi: 10.1161/CIRCRESAHA.121.319374

Figure 4: Vascular ADAR1 was essential for AAA formation.

Figure 4:

A, A graphic illustration of the mouse aortic transplant. Donor aortic segment was anastomosed to recipient abdominal aorta in an end-to-side manner. Recipient mice were then infused with Ang II (1000 ng/kg/min) for 28 days (generated by Biorender). B, Representative transverse ultrasound images of abdominal aorta 28 days after the transplantation. Red dash circles: donor aortas; Green dash circles: recipient mice’s own aortas. C, Maximal external diameters of donor aortas were measured by ultrasound imaging. One-way analysis of variance followed by Tukey’s test was performed to determine statistical difference. **P = 2.17E-03 vs. ApoE−/− mouse donor aortas, n=6, Mann-Whitney test (two-sided). D-E, Representative images of hematoxylin and eosin (H&E) (D) or Verhoeff’s elastic (EVG) staining (E) of the transplanted aortas with aneurysm. The areas in the black box were shown with a higher magnification in the lower part of the panel. Black arrowhead: donor aortas; Black arrow: recipient’s own aortas; Green arrowhead: elastic lamellae breaks. Scar bar= 100 μm. F: Quantification of elastin break indexes in the donor aortas of Ang II-infused ApoE−/− and ADAR1+/−;ApoE−/− recipient mice. Kruskal-Wallis test followed by Dunn’s post-hoc test was performed. **P = 2.165e-3 vs. ApoE−/− mouse donor aortas, n=6, Mann-Whitney test (two-sided). G, Prevalence of AAA. AAA was defined as a percentage increase in aortic diameter ≥100%. Unpaired Student t test was performed. **P = 1.72e-4 vs. ApoE−/− mouse donor aortas, n=6, Fisher’s exact test (two-sided).