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. 2021 Oct 25;12:754287. doi: 10.3389/fpls.2021.754287

Figure 1.

Figure 1

(A) Protease activity and the degree of protein hydrolysis (DH) in ungerminated (0 h) and sprouted lentil and fava bean seeds at different germination times (96 and 144 h). Data are the mean ± SD of the three replicates. Different uppercase letters denote statistical differences among legumes at the same germination time (p ≤ 0.05 post-hoc Duncan's test). (B) A pattern of proteolysis in ungerminated seeds (0 h) and sprouts of lentil and fava bean at different germination times (96 and 144 h). Representative Coomasie-blue stained gels. 1: Unknown protein, 164 kDa; 2: Lipoxygenase isoform 1/Linoleate-9s-lipoxygenase-3 isoform 1, 111 kDa; 3: Lipoxygenase isoform 2, 93 kDa; 4: Convicilin, 68 kDa; 5: Vicilin, 53 kDa; 6: Vicilin, 48 kDa; 7: Vicilin precursor/Legumin β, 20kDa; 8: 2S albumin, 15 kDa; 9: 2S albumin, 4 kDa; 10: Unknown protein, 115 kDa; 11: Lipoxygenase isoform 1, 107 kDa; 12: Lipoxygenase isoform 2, 90 kDa; 13: Convicilin, 66 kDa; 14: Legumin, 35 kDa; 15: 2S albumin, 13 kDa; 16: 2S albumin/Albumin 1, 11kDa; 17: Unknown protein, 170 kDa; 18: Lipoxygenase isoform 1, 109 kDa; 19: Lipoxygenase isoform 2, 91 kDa; 20: Convicilin, 67 kDa; 21: Convicilin, 61 kDa; 22: Legumin, 42 kDa; 23: Legumin, 39 kDa; 24: Legumin, 31 kDa; 25: Vicilin precursor, 19 kDa; 26: Linoleate-9s-lipoxygenase-3 isoform 2, 94 kDa; 27: Convicilin, 79 kDa; 28: Heat shock protein, 70 kDa; 29: Convicilin, 54 kDa; 30: Legumin β, 16 kDa; 31: Albumin 1, 14 kDa; 32: Unknown peptide, 6 kDa; ST, Molecular weight marker. (C) The distribution of protein content between proteins >30 kDa, peptides 3–30 kDa, peptides <3 kDa, and FAA in dry seeds and sprouted lentil and fava bean at different times of germination (96 and 144 h). Data are the mean ± SD of the three replicates. Different lowercase letters indicate statistical differences among different germination endpoints (p ≤ 0.05 post-hoc Duncan's test). Different uppercase letters denote statistical differences among legumes at the same germination time (p ≤ 0.05 post-hoc Duncan's test). FAA, free amino acids; GZL, gray zero-tannin lentil; BL, beluga lentil; DL, dehulled red lentil; ST, molecular weight standard; ZF, low tannin/zero vicine–convicine fava bean.