Table 1.
List of primer pairs used for RT-qPCR analyses. For relative quantification of mRNA levels, the house-keeping genes were β-actin (Actb) and Cyclophilin (Ppid) . The primer sequence, the predicted amplicon size, and the primer efficiency are indicated. bp base pairs, F forward, R reverse
| Gene | Primer sequence | Amplicon size (bp) | Primer efficiency |
|---|---|---|---|
| Actb-F | cccgcgagtacaaccttct | 72 | 1.000 |
| Actb-R | cgtcatccatggcgaact | ||
| Casp3-F | ccgacttcctgtatgcttactcta | 70 | 1.000 |
| Casp3-R | catgacccgtcccttgaa | ||
| Casp8-F | agagcctgagggaaagatgtc | 72 | 1.000 |
| Casp8-R | tcacatcatagttcacgccagt | ||
| Casp9-F | cgtggtggtcatcctctctc | 81 | 1.000 |
| Casp9-R | gagcatccatctgtgccata | ||
| Cd68-F | ctcacaaaaaggctgccact | 60 | 1.000 |
| Cd68-R | ttccggtggttgtaggtgtc | ||
| Iba1-F | ctccgaggagacgttcagtt | 96 | 0.855 |
| Iba1-R | tttttctcctcatacatcagaatcatcagaat | ||
| Il1b-F | tgtgatgaaagacggcacac | 70 | 1.000 |
| Il1b-R | cttcttctttgggtattgtttgg | ||
| Il6-F | cctggagtttgtgaagaacaact | 142 | 1.000 |
| Il6-R | ggaagttggggtaggaagga | ||
| Myd88-F | atgaactgaaggaccgcatc | 127 | 1.000 |
| Myd88-R | cccagttcctttgtctgtgg | ||
| Nfκb-F | ctggcagctcttctcaaagc | 70 | 0.960 |
| Nfκb-R | ccaggtcatagagaggctcaa | ||
| Ppid-F | tgctggaccaaacacaaatg | 88 | 1.000 |
| Ppid-R | cttcccaaagaccacatgct | ||
| Tmem119-F | ttctggctgctactcagaacc | 68 | 1.000 |
| Tmem119-R | ttttgttccctccccactg | ||
| Tgfb-F | cctggaaagggctcaacac | 99 | 1.000 |
| Tgfb-R | tgccgtacacagcagttctt | ||
| Tlr2-F | tgctatgatgcctttgtttcc | 60 | 1.000 |
| Tlr2-R | catgaggttctccacccaat | ||
| Tlr3-F | cttgtcatcaaatccacttaaagagt | 70 | 1.000 |
| Tlr3-R | gaggacgaataacttgccaatc | ||
| Tlr4-F | ccttgagaaagtggagaagtcc | 61 | 1.000 |
| Tlr4-R | gctaagaaggcgatacaattcg | ||
| Tnfa-F | gcccagaccctcacactc | 99 | 1.000 |
| Tnfa-R | ccactccagctgctcctct |