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. 2021 Nov 8;11:21869. doi: 10.1038/s41598-021-01248-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Relative mRNA expression of tight junction protein-encoding genes in the caecum of FRL rats. (A) Relative gene expression of ocln in rats. (B) Relative gene expression of cldn3 in rats. Gene expression was normalized against gene expression of Gapdh using the ΔΔCt method. FMT-MDD: Faecal microbiota transplantation from patients with MDD into rats. FMT-Healthy: Faecal microbiota transplantation from healthy individuals into rats. FMT-MDD-Ser: Faecal microbiota transplantation from patients with MDD into rats combined with treatment with sertraline. CON-Auto: Rats receiving auto-transplantations. CON-H2O: Rats receiving demineralised water.