Table 3.

Overview of primers.

Gene target	Sequence accenssion number	Forward primer	Reverse primer	Product size
Ocln	NM_031329	5′-AACCGACTACACGACAGGTG-3′	5′-AGCCATGTACTCTTCGCTCTC-3′	96
Cldn3	NM_031700	5′-GAATGGACAAAGACACCTCGC-3′	5′-CCACTATGAGCCTTCTGGCTG-3′	129
Gapdh	NM_017008 XM_216453	5′-AGTGCCAGCCTCGTCTCATA-3′	5′-GGTAACCAGGCGTCCGATAC-3′	77

Targets include the genes for Occludin (Ocln), Claudin-3 (Cldn3), and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH, Gapdh).