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. 2021 Sep 1;125(10):1377–1387. doi: 10.1038/s41416-021-01528-1

Fig. 4. Combination of apalutamide with radiation does not induce an early apoptotic response.

Fig. 4

Analysis of apoptosis markers of 22Rv1, PC3, and DU145 prostate cancer cell lines, exposed to radiation (4 Gy for 22Rv1 and DU147 and 10 Gy for PC3): a typical images of apoptotic cells stained with Annexin (green fluorescence), evident 4 days after exposure to radiation; b quantification of Annexin fluorescence intensity in prostate cancer cells exposed to radiation; c lack of expression of Annexin in prostate cancer cells, 24 h after exposure to radiation and apalutamide; d, e western blot images and quantification of cleaved PARP protein expression, 24 h after exposure of 22Rv1 to radiation, Apalutamide, their combination and docetaxel (used as control).