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. 2021 Nov 9;7:108. doi: 10.1038/s41421-021-00346-2

Fig. 1. SARS-CoV-2 infection of human distal lung organoids and its serial cellular responses.

Fig. 1

a Representative microscopy images of tissue-derived hDLOs. Scale bar, 100 μm. b Immunofluorescence staining of different cell markers in hDLOs. Scale bar, 50 μm. c Representative images of hDLOs upon SARS-CoV-2 infection at 3 dpi with indicated MOIs. Yellow arrows indicate the cytopathic organoids caused by SARS-CoV-2. Scale bar, 100 μm. d Immunofluorescence staining of different cell markers and SARS-CoV-2-N antibodies in mock or SARS-CoV-2-infected hDLOs at 3 dpi at MOI = 0.01 and 0.2. Scale bar, 50 μm. The data were repeated for three times. e Heatmap of differentially expressed genes in SARS-CoV-2-infected hDLOs compared to mock infection. f GO analysis of differentially expressed genes in SARS-CoV-2-infected hDLOs at 2, 3, and 4 dpi. The x-axis represents −log10 (P value) for the enrichment analysis. g Variation trends of main pathways along different time points were shown.