Table 2.
Transfer rates of phages, plasmids and chromosomal markers.
| Phage titre (PFU per ml) | Phage transfer (lysogenisation) | Chromosome transfer (phage-mediated lateral transduction) | Plasmid pJP2511 transfer (phage-mediated generalised transduction) | ||||
|---|---|---|---|---|---|---|---|
| Strain | Mean (±SD) | Mean transduction titre (TrU/ml; ±SD) | Transfer frequency (TE/donor)a | Mean transduction titre (TrU/ml; ±SD) | Transfer frequency (TE/donor) | Mean transduction titre (TrU/ml; ±SD) | Transfer frequency (TE/donor) |
| Staphylococcus aureus | |||||||
| JP20844 (φ11) | 6.00 × 108 (±1.83 × 108) | 3.00 × 107 (±2.31 × 107) | 4.62 × 10−1 | 5.00 × 105 (±1.15 × 104) | 7.69 × 10−3 | 2.00 × 105 (±1.15 × 105) | 3.08 × 10−3 |
| JP20846 (80α) | 1.15 × 109 (±7.23 × 108) | 1.10 × 106 (±6.16 × 105) | 1.69 × 10−2 | 3.25 × 105 (±2.63 × 105) | 5.00 × 10−3 | 5.50 × 104 ( ± 5.77 × 103) | 8.46 × 10−4 |
| Salmonella Typhimurium | |||||||
| JP22210 (P22) | 2.03 × 108 (±1.10 × 108) | 1.57 × 108 (±8.33 × 107) | 1.567 | ND | ND | ND | ND |
ND not determined.
Source data are provided as a Source Data file.
Data are the mean values of four (φ11 and 80α) or three (P22) independent experiments.
aTE, transfer events. TE per donor cell = transductant units (TrU) per ml/6.5 × 107 CFU per ml for S. aureus phages, or transductant units (TrU) per ml/1.0 × 108 CFU per ml for S. Typhimurium phage P22.