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. 2021 Nov 8;11:21874. doi: 10.1038/s41598-021-01503-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Scanning electron microscopy of cornea demonstrated that the microvilli of corneal epithelium were well-preserved in the non-dry control (A) but were mostly lost in the dry eye mice (B). Topical application of ADSC-CM (C), < 10 kD fraction of ADSC-CM (E), or < 3 kD fraction of ADSC-CM (G) preserved the microvilli best from dry damage. In contrast, those treated with topical > 10 kD fraction of ADSC-CM (D) or > 3 kD (F) did not have good surfaces covered by microvilli. Magnification: ×25,000 (N = 3).