Table 4.
Ingredients, chemical composition, and fatty acid profile of the experimental diets.
| Item | Diets1 | |||
|---|---|---|---|---|
| C | O | SD | FD | |
| Ingredients (g/kg DM) | ||||
| Barley | 390 | 385 | 342 | 354 |
| Soybean meal | 170 | 168 | 149 | 154 |
| Dehydrated alfalfa | 400 | 395 | 351 | 363 |
| Freeze dried N. oceanica | – | – | – | 92 |
| Spray dried N. oceanica | – | – | 123 | – |
| Nannochloropsis sp. Oil | – | 12 | – | – |
| Calcium carbonate | 13 | 13 | 11 | 12 |
| Sodium bicarbonate | 20 | 20 | 18 | 18 |
| Marine salt | 4 | 4 | 4 | 4 |
| Premix2 | 3 | 3 | 3 | 3 |
| Vitamin E | – | 1.67 | 1.67 | 1.67 |
| Chemical composition (g/kg DM) | ||||
| DM | 904 | 907 | 902 | 907 |
| Crude Protein | 191 | 183 | 206 | 211 |
| Ether Extract | 13.3 | 32.5 | 35.2 | 33.9 |
| NDF | 267 | 267 | 232 | 290 |
| ADF | 173 | 174 | 150 | 167 |
| ADL | 30.3 | 28.1 | 26.8 | 31.2 |
| Sugar | 73.2 | 69.4 | 65.5 | 67.9 |
| Starch | 277 | 279 | 247 | 227 |
| Crude Energy (kJ/100 g) | - | - | - | - |
| Ash | 84.6 | 89.9 | 114.1 | 90.8 |
| Total Fatty acids (g/kg DM) | 13.7 | 20.6 | 19.5 | 20.2 |
| FA profile (g/kg DM) | ||||
| 14:0 | n.d | 0.26 | 0.47 | 0.46 |
| 16:0 | 3.49 | 4.74 | 4.66 | 5.27 |
| 16:1c9 | 0.07 | 1.06 | 1.99 | 1.66 |
| 17:0 | 0.09 | 0.07 | n.d | n.d |
| 18:0 | 0.56 | 0.61 | 0.49 | 0.67 |
| 18:1c9 | 2.55 | 2.99 | 2.30 | 2.77 |
| 18:1c11 | 0.10 | 0.19 | 0.14 | 0.14 |
| 18:2n-6 | 5.62 | 6.49 | 5.50 | 5.88 |
| 18:3n-3 | 1.22 | 1.40 | 0.98 | 1.30 |
| 20:4n-6 | n.d | 0.53 | 0.72 | 0.64 |
| 20:5n-3 (EPA) | n.d | 2.14 | 2.20 | 1.39 |
| 22:0 | n.d | 0.12 | 0.07 | n.d |
1Diets (or treatments): C, control; O, Control plus 1.2% Nannochloropsis sp. oil; SD, control plus 12.3% spray-dried Nannochloropsis oceanica; FD, control plus 9.2% freeze-dried N. oceanica. 2Premix composition: Vitamin A, 4,000,000 UI; Vitamin D3, 1,100,000 UI; Vitamin E, 7500 mg/kg; Vitamin B1, 250 mg/kg; Vitamin B2 250 mg/kg; Zinc, 35,000 mg/kg; Iron, 12,500 mg/kg; Manganese, 17,500 mg/kg; Iodine, 200 mg/kg; Cobalt 250 mg/kg; Selenium, 100 mg/kg; Magnesium oxide (excipient) 40,000 mg/kg. n.d. – not detected.