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. Author manuscript; available in PMC: 2022 Jan 29.
Published in final edited form as: Semin Cancer Biol. 2021 May 9;78:90–103. doi: 10.1016/j.semcancer.2021.04.021

Fig. 5.

Fig. 5.

Autochthonous TRAMP tumors harbor LT-LRCs. Eight-week old TRAMP tumors in C57/FVB mice were continuously pulsed with 20 mg/ml BrdU using the slow-releasing Alzet pumps for 19 days (i.e., from Dec. 22 of 2005 to Jan. 10 of 2006) and then the pumps were removed (i.e., termination of pulse; indicated by *). Prostates chased for 1 day, 6 or 8 weeks (wks), or 3 or 6 months were harvested and embedded in paraffin. Serial sections of 5 μm were cut and used in BrdU IHC staining. At least 5 sections were analyzed and BrdU+ cells counted. Shown are the average BrdU+ cells in the dorsal prostate (DP) and ventral prostate (VP), respectively.