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. 2021 Oct 26;15:752958. doi: 10.3389/fnins.2021.752958

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Copyright © 2021 Thomas, Lin, Martinez-Camarillo, Zhu, McLelland, Nistor, Keirstead, Humayun and Seiler.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transplant histology at 2 months (A,B) and 6.5 months (C–E) post-surgery. (A,C) Co-graft area. (B,D) Host retina outside co-graft. (A–D) H&E staining. White asterisks indicate the center of rosettes (spherical arrangement of photoreceptors and other retinal layers around a central lumen). The transplant in (C) has grown around the parylene RPE implant (transplant #3 in Table 3, 197 dps). (E) Bestrophin (gold) and DAPI (blue) shows RPE and parylene in transplant (#2 in Table 3, 195 dps). Blue arrows point to parylene. Red arrows point to hESC-RPE. Yellow arrows point to host RPE. The separation of tissue is processing artifact. Scale bars = 100 μm (A–D); 20 μm (E).