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. 2021 Nov 4;14:5801–5816. doi: 10.2147/JIR.S331020

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© 2021 Zhang et al.

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Mice were intratracheally atomized with 5 mg/kg LPS (stimulation for 6, 12, 18, and 24 h) to observe the changes of tight junction. Occludin protein was detected by immunohistochemistry (A, magnification, × 200; scale bar, 50 μm) and Western blot (E). The ZO-1 protein was detected by immunofluorescence technique (C, magnification, × 200; scale bar, 50 μm) and Western blot (F). (D) Fluorescence intensity analysis of (C). (B, G, and H) are the protein intensity analyses of (A, E and F) respectively. All data are presented as the mean ± SD of three independent experiments. ^#p < 0.05 vs the control group.