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. 2021 Nov 4;14:5801–5816. doi: 10.2147/JIR.S331020

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© 2021 Zhang et al.

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Downregulation MMP-9 expression alleviated tight junction impairment in vivo and in vitro. Occludin was detected by immunohistochemistry (A, magnification, × 200; scale bar, 50 μm) and immunofluorescence (E, magnification, × 200; scale bar, 50 μm). The ZO-1 in mice (C, magnification, × 200; scale bar, 50 μm) and A549 cells (G, magnification, × 200; scale bar, 50 μm) were detected by immunofluorescence. (B) is the protein intensity analysis of (A). (D, F, and H) are the fluorescence intensity analyses of (C, E and G) respectively. All data are presented as the mean ± SD of three independent experiments. ^#p < 0.05 vs the control group, ^#*p < 0.05 vs the LPS group.