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. 2021 Nov 3;10(11):bio058910. doi: 10.1242/bio.058910

Fig. 3.

Fig. 3.

stum 9-3 functions upstream of the S2 cleavage of the Notch receptor. (A) stum 9-3 ISC MARCM clones (green) (labeled with yellow dotted lines, white arrowheads). GFP and DAPI channels are showed separately in black-white. (B) Expression of full-length Notch could not rescue defects observed in stum 9-3 ISC MARCM clones (green) (labeled with yellow dotted lines, white arrowheads). (C,D) Expression of NEXT (C) or NICD (D) could completely suppress ISC over-proliferation observed in stum 9-3 ISC MARCM clones (green) (labeled with yellow dotted lines, white arrowheads). (E) Quantification of the size of ISC MARCM clones in different genotypes indicated. n=25–35. Mean±s.d. is shown, ****P<0.0001. Please note that stum 9-3 ISC clones are deformed, preventing accurate quantification of clone size. In all panels except graphs, GFP is in green, blue indicates DAPI staining for DNA. Scale bars: 20 μm. Yellow arrows correspond to the white arrows, pointing to the cells that the white arrows pointed to.