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Primer sequences for genes amplified in this study. F, forward primer; R, reverse primer.
| Gene | Primer sequence (5ʹ-3ʹ) | Amplified fragment (bp) |
|---|---|---|
| ß-Actin | F: TGCGGGACATCAAGGAGAAG R: AGTTGAAGGTGGTCTCGTGG |
92 |
| claudin | F: GGCCCTTACCTTTCGCCTGA R: GCCTCAGGGCTTGGTGTTCT |
99 |
| occludin | F: ATCAACAAAGGCAACTCT R: GCAGCAGCCATGTACTCT |
74 |
| ZO-1 | F: GCCTCAGGGCTTGGTGTTCT R: GGCCCTTACCTTTCGCCTGA |
87 |
