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. 2021 Oct 26;11:735183. doi: 10.3389/fonc.2021.735183

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Copyright © 2021 Yueh, Lee, Chiang, Chen, Lan, Chen and Hsu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The suppression of NF-κB mediated metastasis ability by imipramine in CL1-5-F4 cells. (A, B) The migration pattern and quantification of gap area after treatment with 100 and 150 μM imipramine are performed by wound healing assay and displayed. (C) The crystal violet staining results of wound healing assay after 20 hr migration are presented. (D–F) The transwell migration, invasion and quantification bar chart after imipramine treatment are displayed. (G) The protein expression of MMP9, VEGF, MCL-1, cFLIP, XIAP and their quantification results are displayed. (^** p < 0.01 vs. 0 μM imipramine; ^## p < 0.01 vs. 100 μM imipramine).