FIGURE 2.

PGLS pathway led to significant activation of PPP in HCC. (A) The representative FACS plot of PGLS^high and PGLS^low HCC cells. (B,C) Western blot of the PGLS and CK18 in PGLS^high and PGLS^low cells from human HCC patients. β-Actin is a loading control. (D) The relative expression of HCC marker genes in PGLS^high and PGLS^low cells (n = 3 replicates). (E) Glycometabolism diagram. (F) The NADP⁺/NADPH ratio in PGLS^high and PGLS^low cells (n = 5 replicates). (G) The percentage of ROS in PGLS^high and PGLS^low cells (n = 5 replicates). (H) The representative FACS plot of PGLS^high and PGLS^low cells for apoptosis analysis. (I) The apoptosis rate of PGLS^high and PGLS^low cells. (J) The expression profile of genes associated with apoptosis pathway between PGLS^high and PGLS^low cells. (K) KEGG analysis of PGLS^high and PGLS^low cells. Data show individual values and mean ± SD. (C,D,F,G,I) Unpaired two-tailed Student t-tests, assessed statistical significance, *p < 0.05, **p < 0.01, ***p < 0.001.