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. 2021 Oct 26;9:753196. doi: 10.3389/fcell.2021.753196

FIGURE 3.

FIGURE 3

PGLS regulated the proliferation and apoptosis of HCC. (A) Transfection with the three siRNAs could effectively knock down the expression of PGLS on Huh7. (B) Quantitative analysis of Western blot in (A). (C) The NADP+/NADPH ratio on Huh7. (D) The percentage of ROS on Huh7. (E) The apoptosis rate on Huh7. (F) Fluorescence micrographs of Huh7 after staining with calcein-AM, PI, and DAPI. (G) Proliferation curve of Huh7 transfected by siRNA-1, siRNA-2, and siRNA-3. (H) Representative field of CFUs, formed by Huh7 treated with siRNA-1, siRNA-2, and siRNA-3. (I) CFU number per field on Huh7. Data show individual values and mean ± SD. (B–E,G,I) Unpaired two-tailed Student t-tests, assessed statistical significance, ***p < 0.001.